Senior Honors Projects, 2010-current

Date of Award

Fall 2015

Document Type

Dissertation/Thesis

Degree Name

Bachelor of Science (BS)

Department

Department of Integrated Science and Technology

Advisor(s)

Stephanie Stockwell

Abstract

Soybean is one of the leading crops grown in the United States. Nitrogen fertilizers are widely used in soybean production to increase crop yields. An alternative to nitrogen fertilizers is the symbiotic relationship between soybean and a species of nitrogen-fixing Gram-negative soil bacteria, Bradyrhizobium japonicum. The bacterium infects the roots of the soybean, forming nodules that perform nitrogen fixation. This study involves analysis of TonB-dependent receptors, intermembrane proteins responsible for iron uptake and, in some cases, symbiosis in B. japonicum. A comparison of sequence alignments and 3D structure predictions was used to identify potential symbiosis-specific structural domains within TonB-dependent receptors as targets for site-directed mutagenesis.

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