Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Date of Graduation

Fall 2011

Document Type


Degree Name

Master of Science (MS)


Department of Biology


The nascent polypeptide-associated complex (NAC) is a highly conserved protein complex known to play an important role in the development of metazoan organisms, but its molecular function is not well understood. Recent evidence from experiments using Saccharomyces cerevisiae as model supported the hypothesis that the NAC is either a chaperone or a component of the cytosolic chaperone network that interacts with nascent peptides emerging from the ribosome. We tested this model in C. elegans and found that the homologues of the NAC , icd-1 and icd-2, behave like chaperones in the worm. Lack of icd-1 or icd-2 altered the worms stress response to heat and led to a dramatic up-regulation of hsp-4; the homologue of the human ER chaperone BiP. Worms lacking the ER stress signalling protein xbp-1 generated a higher proportion of defective embryos and had a lower survival rate compared to wildtype populations during icd-1(RNAi). Furthermore, icd-1(RNAi) increased the size of lysosomes in wildtype worms and embryo gut cells, indicating an up-regulation of ER-mediated autophagy. These results suggest that disruption of the NAC by RNAi causes ER stress in the worm and is likely the cause of embryonic apoptosis that was previously observed in the worm.

Included in

Biology Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.