Preferred Name

Andy Allen

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

ORCID

https://orcid.org/0000-0001-8814-089X

Date of Graduation

5-7-2020

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Department of Kinesiology

Advisor(s)

Christopher J. Womack

Stephanie P. Kurti

Trent A. Hargens

Abstract

Abstract

Background: There is a strong association between factor VIII (FVIII) and presence of cardiovascular disease (CVD). Exercise is a known trigger for myocardial infarction, and the majority of ischemic events from physical stress occur due to an occlusive thrombus. FVIII increases acutely with exercise and remains elevated hours after exercise. Furthermore, FVIII is also higher in cigarette smokers, however, it has not been investigated in e-cigarette smokers. The purpose of this study is to identify potential differences in FVIII before exercise, after exercise, and its magnitude of change in e-cigarette users versus non-users.

Methods: Eighteen individuals (8 e-cigarette users, 3 women and 5 men, and 10 non-smokers, 7 women and 3 men; age, 18-32 years old) were recruited to participate in this research study. An incremental test to exhaustion (x̄=12.20 ± 3.20 minutes) was performed on an electronically braked cycle ergometer (Lode Corival cpet; Groningen, Netherlands) to determine the peak rate of oxygen consumption (VO2peak). Ten mL of blood was drawn into a citrate anticoagulant solution from an antecubital vein using clean venipuncture with minimal stasis both prior to and immediately following exercise testing. A commercially available ELISA kit was used to determine plasma concentrations of coagulation FVIII antigen.

Results: There was no significant difference between smokers (N = 8, 4.35 ± 2.09 IU/ml) and nonsmokers (N = 9, 5.02 ± 4.87 IU/ml, p=0.72) for resting FVIII antigen. There was a main effect for exercise in that the post-exercise values (N=10, 7.76 ±1.40 IU/ml) were larger than pre-exercise values (N=10, 3.82 ± 0.65, p=0.003). However, there was no main effect for smoking and no smoking x exercise interaction (post-exercise smokers, N=4, 6.67 ± 1.46, post-exercise non-smokers, N= 7, 8.39 ± 5.81, p=0.58).

Conclusion: Our preliminary data suggests that e-cig use does not significantly affect FVIII or the FVIII response to exercise.

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