Senior Honors Projects, 2010-current

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

Date of Award

Spring 2019

ORCID

https://orcid.org/0000-0002-1697-2619

Document Type

Thesis

Degree Name

Bachelor of Science (BS)

Department

Department of Integrated Science and Technology

Advisor(s)

Robert L. McKown

Abstract

Current diagnostics of dry eye disease are unable to identify the multiple complex causes of dry eye, thereby limiting the proper treatments of the disease. The tear glycoprotein lacritin has been reported to be decreased in dry eye patients, specifically those with Sjӧgren’s syndrome associated dry eye. Therefore, the use of lacritin in a diagnostic test for dry eye could improve the current diagnostics available. Western blot assays have revealed the presence of an active lacritin monomer as well as an inactive tissue transglutaminase cross-linked polymer in human tears. A third isoform of lacritin, the splice variant lacritin-c, has also been detected in human tears. Using a diagnostic multiplex western blot assay, the three lacritin isoforms were quantitated in forty samples of human tears from twenty adults and analyzed using JMP software. This provided a baseline distribution of percent lacritin in human tears that will be used in future lacritin clinical trials.

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